PXD024808 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Profile of the SARS-CoV-2 RNA interactome |
Description | SARS-CoV-2 is an RNA virus whose success as a pathogen relies on its ability to repurpose host RNA-binding proteins (RBPs) to form its own RNA interactome. Here, we developed and applied a robust ribonucleoprotein capture protocol to uncover the SARS-CoV-2 RNA interactome. We report 109 host factors that directly bind to SARS-CoV-2 RNAs including general antiviral factors such as ZC3HAV1, TRIM25, and PARP12. Applying RNP capture on another coronavirus HCoV-OC43 revealed evolutionarily conserved interactions between viral RNAs and host proteins. Network and transcriptome analyses delineated antiviral RBPs stimulated by JAK-STAT signaling and proviral RBPs responsible for hijacking multiple steps of the mRNA life cycle. By knockdown experiments, we further found that these viral-RNA-interacting RBPs act against or in favor of SARS-CoV-2. Overall, this study provides a comprehensive list of RBPs regulating coronaviral replication and opens new avenues for therapeutic interventions. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-28 |
AnnouncementXML | Submission_2021-04-27_22:03:44.125.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yeon Choi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-17 11:19:58 | ID requested | |
⏵ 1 | 2021-04-27 22:03:44 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: SARS-CoV-2, HCoV-OC43, Vero, HCT-8, RNA interactome, RBP, RNP |
Contact List
V. Narry Kim |
contact affiliation | Center for RNA Research, Institute for Basic Science, Seoul, Republic of Korea. School of Biological Sciences, Seoul National University, Seoul, Republic of Korea. |
contact email | narrykim@snu.ac.kr |
lab head | |
Yeon Choi |
contact affiliation | Seoul National University |
contact email | kitechoi9599@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024808
- Label: PRIDE project
- Name: Profile of the SARS-CoV-2 RNA interactome