PXD024701

PXD024701 is an original dataset announced via ProteomeXchange.

Title	TMT-based quantitative proteomics analysis reveals the function of ester cyclase in the rice false smut fungus Ustilaginoidea virens
Description	Rice false smut is a world-wide fungal disease caused by Ustilaginoidea virens. In this study, we found an ester cyclase, UvEC1, was significantly up-regulated during U. virens infection. UvEC1 contained a ketone cyclase Snoal-like polyketide cyclase domain and the functions of this important class of proteins in plant fungal pathogens remain unclear. Deletion of UvEC1 caused defects in vegetative growth and conidiation. UvEC1 is also required for response to hyperosmotic and oxidative stresses, and maintenance of cell wall integrity. Importantly, the ∆UvEC1 mutants exhibited reduced virulence. We performed an TMT-based quantitative proteomic analysis to identify differentially expressed proteins (DEPs) between ∆UvEC1-1 mutant and the wild type HWD-2. Proteomic data have revealed that UvEC1 have various effects on metabolism process, localization, catalytic activity, binding and spliceosome. Taken together, our findings suggest that UvEC1 is critical for development and virulence of U. virens.
HostingRepository	PRIDE
AnnounceDate	2021-04-15
AnnouncementXML	Submission_2021-04-15_00:25:31.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pei zhangxin
SpeciesList	scientific name: Ustilaginoidea virens; NCBI TaxID: 1159556;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-12 02:44:42	ID requested
⏵ 1	2021-04-15 00:25:31	announced

Dataset with its publication pending

submitter keyword: Ustilaginoidea virens

rice false smut

ester cyclase

virulence

gene function

LU Zheng
contact affiliation	Huazhong Agricultural University
contact email	luzheng@mail.hzau.edu.cn
lab head
Pei zhangxin
contact affiliation	17671788121
contact email	Peizhangxin@webmail.hzau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024701
     1. Label: PRIDE project
     2. Name: TMT-based quantitative proteomics analysis reveals the function of ester cyclase in the rice false smut fungus Ustilaginoidea virens