<<< Full experiment listing

PXD024605

PXD024605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleToo Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper
DescriptionCapsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions are paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense x frutescens). AMP prediction algorithms reveal 115 putative AMPs within the Capsicum chinense genome of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of post-translational modifications and disulfide bond connectivity. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibit low µM antimicrobial activity against gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to more fully characterize plant AMP profiles.
HostingRepositoryPRIDE
AnnounceDate2021-08-13
AnnouncementXMLSubmission_2021-08-13_08:31:20.065.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLeslie Hicks
SpeciesList scientific name: Capsicum chinense x Capsicum frutescens; NCBI TaxID: 936530;
ModificationList2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; TripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-09 01:30:22ID requested
12021-08-13 08:31:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ghost pepper, Capsicum chinense, Capsicum frutescens, Mass spectrometry, peptidomics
Contact List
Leslie M. Hicks
contact affiliationDepartment of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States
contact emaillmhicks@email.unc.edu
lab head
Leslie Hicks
contact affiliationUniversity of North Carolina at Chapel Hill
contact emaillmhicks@unc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024605
PRIDE project URI
Repository Record List
[ + ]