PXD024605 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper |
Description | Capsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions are paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense x frutescens). AMP prediction algorithms reveal 115 putative AMPs within the Capsicum chinense genome of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of post-translational modifications and disulfide bond connectivity. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibit low µM antimicrobial activity against gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to more fully characterize plant AMP profiles. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-13 |
AnnouncementXML | Submission_2021-08-13_08:31:20.065.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Leslie Hicks |
SpeciesList | scientific name: Capsicum chinense x Capsicum frutescens; NCBI TaxID: 936530; |
ModificationList | 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-09 01:30:22 | ID requested | |
⏵ 1 | 2021-08-13 08:31:20 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Ghost pepper, Capsicum chinense, Capsicum frutescens, Mass spectrometry, peptidomics |
Contact List
Leslie M. Hicks |
contact affiliation | Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States |
contact email | lmhicks@email.unc.edu |
lab head | |
Leslie Hicks |
contact affiliation | University of North Carolina at Chapel Hill |
contact email | lmhicks@unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024605
- Label: PRIDE project
- Name: Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper