PXD024599

PXD024599 is an original dataset announced via ProteomeXchange.

Title	Dynamic methylation of histone H3K18 in differentiating Theileria parasites
Description	Lysine methylation on histone tails impacts genome regulation and cell fate determination in many developmental processes. Apicomplexa intracellular parasites cause major diseases and they have developed complex life cycles with fine-tuned differentiation events. Yet, apicomplexa genomes have few transcription factors and little is known about their epigenetic control systems. Tick-borne Theileria apicomplexa species have relatively small, compact genomes and a remarkable ability to transform leukocytes in their bovine hosts. Here we report enriched H3 lysine 18 monomethylation (H3K18me1) on the gene bodies of repressed genes in Theileria macroschizonts. Differentiation to merozoites (merogony) led to decreased H3K18me1 in parasite nuclei. Pharmacological manipulation of H3K18 acetylation or methylation impacted parasite differentiation and expression of stage-specific genes. Finally, we identified a parasite SET-domain methyltransferase (TaSETup1) that can methylate H3K18 and represses gene expression. Thus, H3K18me1 emerges as an important epigenetic mark which controls gene expression and stage differentiation in Theileria parasites.
HostingRepository	PRIDE
AnnounceDate	2021-03-25
AnnouncementXML	Submission_2021-03-25_08:23:36.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laurent Lignières
SpeciesList	scientific name: Theileria annulata strain Ankara; NCBI TaxID: 353154;
ModificationList	monomethylated residue; dimethylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-09 01:28:00	ID requested
⏵ 1	2021-03-25 08:23:36	announced

Dataset with its publication pending

submitter keyword: Theileria, host parasite interactions, epigenetic, H3K18, TaSETup1

Guillaume Chevreux
contact affiliation	Proteomic Facility, Institut Jacques Monod, UMR7592 (CNRS and Paris University)
contact email	guillaume.chevreux@ijm.fr
lab head
Laurent Lignières
contact affiliation	Proteomic facility of Institut Jacques Monod - CNRS & Université de Paris
contact email	laurent.lignieres@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD024599

PRIDE project URI

• PRIDE
  1. PXD024599
     1. Label: PRIDE project
     2. Name: Dynamic methylation of histone H3K18 in differentiating Theileria parasites