PXD024588

PXD024588 is an original dataset announced via ProteomeXchange.

Title	Protein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus)
Description	Proteins play an important role in many reproductive functions such as sperm maturation and physiology, sperm transit in the female genital tract or sperm-oocyte interaction. However, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained voluntarily from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using a LC-MS/MS and a list with the gene symbols corresponding to each protein was submitted to DAVID database. A total of 423 and 307 proteins were identified in spermatozoa and seminal plasma, respectively, and the samples shared 111 proteins. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The top-5 most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GST3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this proteomic study provides the first characterization of proteomic expression patterns in cetacean sperm and seminal plasma, opening a window to future research on the discovery of biomarkers, analysis of conservation capacity or additional applications in the field of assisted reproduction technologies.
HostingRepository	PRIDE
AnnounceDate	2021-06-14
AnnouncementXML	Submission_2021-06-14_01:00:44.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Tursiops truncatus truncatus; NCBI TaxID: 2663531;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-08 22:52:10	ID requested
⏵ 1	2021-06-14 01:00:45	announced

Dataset with its publication pending

submitter keyword: Cetacean

Proteomic

Semen

Sperm cells

Seminal plasma

Mª José Izquierdo-Rico
contact affiliation	Department of Cell Biology and Histology, Faculty of Medicine, Campus Mare Nostrum,University of Murcia, 30100 Murcia, IMIB, Spain
contact email	mjoseir@um.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD024588

PRIDE project URI

• PRIDE
  1. PXD024588
     1. Label: PRIDE project
     2. Name: Protein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus)