PXD024579

PXD024579 is an original dataset announced via ProteomeXchange.

Title	Ovalbumin antigen-specific activation of T cell receptor closely resembles antibody stimulation as revealed by BOOST phosphotyrosine proteomics
Description	Here we provide a framework to qualtitatively evaluate the tyrosine phosphorylation-mediated signaling pathways triggered by antigen-independent antibody and antigen-specific OVA peptide-MHC using Jurkat T cells expressing OT-1 T cell receptors. Our data suggest that pTyr-mediated regulatory axis triggered by OVA antigen-specific activation of TCR closely resembled that of antigen-independent stimulation using anti-TCR antibody, albeit OVA could likely induce a relatively stronger signaling effect. While data from this study do not invalidate previous studies of T cell signaling using antibody-based stimulation, our data revealed potential advantages of using peptide-MHC tetramers in studying T cell signaling. Antigen-specific activation of the OT-1 TCR using a panel of peptide-MHC tetramers (OVA, T4 and G4) generated data that correlates well with the corresponding binding affinity of the peptide-MHC, consistent with predicted signaling strength. Importantly, the apparent correlation between signaling strength and peptide-MHC affinity enables us to fine-tune the signaling strength of T cell stimulation in future studies, allowing a more precise control of the experimental parameter instead of a more binary antibody-based activation. Overall, we demonstrate the utility of BOOST to reveal new biological insights in the immune system.
HostingRepository	PRIDE
AnnounceDate	2021-04-27
AnnouncementXML	Submission_2021-04-26_22:12:27.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xien Yu Chua
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-08 01:04:21	ID requested
⏵ 1	2021-04-26 22:12:27	announced

Dataset with its publication pending

submitter keyword: T cell receptor, T cell signaling, OT-1, BOOST, TMT, phosphotyrosine proteomics

Arthur R Salomon
contact affiliation	Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA
contact email	art@drsalomon.com
lab head
Xien Yu Chua
contact affiliation	Brown University
contact email	xien_yu_chua@brown.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD024579

PRIDE project URI

• PRIDE
  1. PXD024579
     1. Label: PRIDE project
     2. Name: Ovalbumin antigen-specific activation of T cell receptor closely resembles antibody stimulation as revealed by BOOST phosphotyrosine proteomics