PXD024532
PXD024532 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis |
Description | The shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis. |
HostingRepository | MassIVE |
AnnounceDate | 2021-04-06 |
AnnouncementXML | Submission_2021-04-06_09:10:50.748.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mark Adamo |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl; Oxidation; Phospho |
Instrument | Orbitrap Fusion Lumos; Orbitrap Fusion; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-03-04 16:38:03 | ID requested | |
⏵ 1 | 2021-04-06 09:10:51 | announced |
Publication List
no publication |
Keyword List
submitter keyword: shugoshin, centromere, cohesin, mitosis, meiosis, hSgo1, PP2A-B56, coiled coil, cohesion, PP2A, B56, Cdk1 |
Contact List
Arminja Kettenbach | |
---|---|
contact affiliation | The Geisel School of Medicine at Dartmouth |
contact email | arminja.n.kettenbach@dartmouth.edu |
lab head | |
Mark Adamo | |
contact affiliation | Dartmouth College |
contact email | mark.e.adamo@dartmouth.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087003/ |