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PXD024532

PXD024532 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA highly conserved pocket on PP2A-B56 is required for hSgo1 binding and cohesion protection during mitosis
DescriptionThe shugoshin proteins are universal protectors of centromeric cohesin during mitosis and meiosis. The binding of human hSgo1 to the PP2A-B56 phosphatase through a coiled coil (CC) region mediates cohesion protection during mitosis. Here we undertook a structure function analysis of the PP2A-B56-hSgo1 complex, revealing unanticipated aspects of complex formation and function. We establish that a highly conserved pocket on the B56 regulatory subunit is required for hSgo1 binding and cohesion protection during mitosis in human somatic cells. Consistent with this, we show that hSgo1 blocks the binding of PP2A-B56 substrates containing a canonical B56 binding motif. We find that PP2A-B56 bound to hSgo1 dephosphorylates Cdk1 sites on hSgo1 itself to modulate cohesin interactions. Collectively our work provides important insight into cohesion protection during mitosis.
HostingRepositoryMassIVE
AnnounceDate2021-04-06
AnnouncementXMLSubmission_2021-04-06_09:10:50.748.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMark Adamo
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation; Phospho
InstrumentOrbitrap Fusion Lumos; Orbitrap Fusion; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-04 16:38:03ID requested
12021-04-06 09:10:51announced
Publication List
no publication
Keyword List
submitter keyword: shugoshin, centromere, cohesin, mitosis, meiosis, hSgo1, PP2A-B56, coiled coil, cohesion, PP2A, B56, Cdk1
Contact List
Arminja Kettenbach
contact affiliationThe Geisel School of Medicine at Dartmouth
contact emailarminja.n.kettenbach@dartmouth.edu
lab head
Mark Adamo
contact affiliationDartmouth College
contact emailmark.e.adamo@dartmouth.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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