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PXD024504

PXD024504 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGlobal coordination of metabolic pathways in Escherichia coli by active and passive regulation
DescriptionIn this study, we identify the regulatory mechanisms that coordinate catabolism and anabolism in the bacterium Escherichia coli. Integrating protein, metabolite, and metabolic flux changes in genetically implemented gradual catabolic or anabolic limitations, we show that a combination of global and local mechanisms coordinates the response to metabolic limitations. To allocate proteomic resources between catabolism and anabolism, E. coli uses a simple global gene regulatory program. Surprisingly, this program is largely implemented by a single transcription factor Crp, which not only directly activates the expression of catabolic enzymes, but also indirectly reduces the expression of anabolic enzymes by passively sequestering cellular resources needed for their synthesis. However, this regulatory program rarely controls metabolic fluxes alone; instead, fluxes are further adjusted locally through passive changes in metabolite concentrations. Together, these mechanisms constitute a limited but effective global regulatory program that coarsely partitions resources between different parts of metabolism while ensuring the robust coordination of individual metabolic reactions.
HostingRepositoryMassIVE
AnnounceDate2021-03-10
AnnouncementXMLSubmission_2021-03-10_09:20:23.725.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVadim Patsalo
SpeciesList scientific name: Escherichia coli K-12; NCBI TaxID: 83333;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-03 11:39:06ID requested
12021-03-10 09:20:24announced
Publication List
no publication
Keyword List
submitter keyword: cAMP, gene expression, growth laws, regulation, cyclic AMP, gene regulation, transcription
Contact List
Karl Kochanowski
contact affiliationETH Zurich
contact emailkarl.kochanowski@gmail.com
lab head
James R. Williamson
contact affiliationThe Scripps Research Institute
contact emailjrwill@scripps.edu
lab head
Uwe Sauer
contact affiliationETH Zurich
contact emailsauer@imsb.biol.ethz.ch
lab head
Terence Hwa
contact affiliationUC San Diego
contact emailhwa@ucsd.edu
lab head
Vadim Patsalo
contact affiliationScripps Research
contact emailpatsalov@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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