PXD024470
PXD024470 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Succinylome-controlled antibiotic resistance in MRSA - Succinylome |
Description | A sucC and a sucD mutant from the Nebraska Transposon Mutant Library (NTML, Strain NE569 and NE1770) were found to be susceptible to cefoxitin and oxacillin when other mutations in TCA cycle genes did not affect their resistance to these antibiotics. The SucC strain (Strain NE569) accumulated Succinyl CoA, which is known to result in higher succinylation rate. We compared the proteome of the sucC mutant in exponential and stationary stages to the one of the WT strain JE2. The global proteome of the following strains were analyzed by TMT-labelled proteomics: Early exponential JE2 (3Hrs), Exponential JE2 (4Hrs), Exponential SucC (4Hrs), Stationary JE2 (10Hrs), Stationary SucC (12Hrs). For each condition, the bacterial pellet was collected and washed twice with PBS. The proteins were extracted by bead beating, 1000 ug of proteins were reduced with DTT, alkylated with IAA and digested with 1:50 (trypsin:protein, w:w) for 3 Hr at 37 C. 100ug of peptides were labelled with TMT11Plex reagents. The samples were pooled and enriched for Succinyl-lysine containing peptides using the PTMScan Succinyl-Lysine Motif Enrichment Kit following the supplier's instructions. The retained fraction was cleaned-up by C18 solid phase extraction. The peptides were concentrated in 15 ul and 5 ul were analyzed by reverse-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Waters nanoEquity UPLC system coupled with a QExactive HF-X mass spectrometer. |
HostingRepository | MassIVE |
AnnounceDate | 2021-06-14 |
AnnouncementXML | Submission_2021-06-14_15:27:55.799.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Matthew Monroe |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | Carbamidomethyl; TMT6plex; Oxidation |
Instrument | Q Exactive HF-X |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-03-02 11:38:29 | ID requested | |
⏵ 1 | 2021-06-14 15:27:56 | announced |
Publication List
no publication |
Keyword List
submitter keyword: proteomics, antibiotic resistance, MRSA, SucC, succinyl-lysine, succinylome |
Contact List
James P. O'Gara | |
---|---|
contact affiliation | National University of Ireland, Galway |
contact email | jamesp.ogara@nuigalway.ie |
lab head | |
Geremy Clair | |
contact affiliation | Pacific Northwest National Laboratory |
contact email | geremy.clair@pnnl.gov |
lab head | |
Matthew Monroe | |
contact affiliation | Pacific Northwest National Laboratory |
contact email | matthew.monroe@pnnl.gov |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086971/ |