PXD024454

PXD024454 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide profiling of the selectivity of diverse electrophiles
Description	Targeted covalent inhibitors (TCIs) are established as powerful entities in drug discovery, but their application has so far mainly been limited to addressing cysteine. Competitive, residue-specific proteomics has been instrumental to streamline covalent drug discovery targeting cysteine. Several probes have recently been described to monitor other amino acids using this technology and many more electrophiles exist to modify proteins. Nevertheless, a direct, proteome-wide comparison of the amino acid selectivity of diverse probes is still entirely missing. Here, we developed a completely unbiased workflow to analyse electrophile selectivity proteome-wide and applied it to directly compare 54 alkyne probes containing diverse reactive groups. In this way, we verified and newly identified probes to monitor nine different amino acids as well as the N-terminus proteome-wide. This selection e.g. includes the first probes to globally monitor tryptophan, histidine and arginine as well as novel tailored probes for methionine, aspartate and glutamate.
HostingRepository	PRIDE
AnnounceDate	2025-08-10
AnnouncementXML	Submission_2025-08-10_06:20:20.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Hacker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-01 22:36:29	ID requested
⏵ 1	2025-08-10 06:20:20	announced

Dataset with its publication pending

submitter keyword: Covalent Inhibitors, Chemical Proteomics, Activity-based Protein Profiling, isoDTB-ABPP

Stephan M. Hacker
contact affiliation	Technical University Munich, Department of Chemistry, Lichtenbergstrasse 4, 85748 Garching bei Muenchen, Germany
contact email	stephan.m.hacker@tum.de
lab head
Stephan Hacker
contact affiliation	Leiden University
contact email	s.m.hacker@lic.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD024454

PRIDE project URI

• PRIDE
  1. PXD024454
     1. Label: PRIDE project
     2. Name: Proteome-wide profiling of the selectivity of diverse electrophiles