PXD024381 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum |
Description | ost-pathogen interactions are often studied in vitro using primary or immortal cell lines. This set-up avoids ethical problems of animal testing and has the additional advantage of lower costs. However, the influence of cell culture media on bacterial growth and metabolism is not considered or investigated in most cases. To address this question growth and proteome adaptation of Corynebacterium diphtheriae strain ISS3319 were investigated in this study. Bacteria were cultured in standard growth medium, cell culture medium and fetal calf serum. Mass spectrometric analyses and label-free protein quantification hint to an increased bacterial pathogenicity when grown in cell culture medium as well as an influence of the growth medium on the cell envelope. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-12 |
AnnouncementXML | Submission_2021-03-12_00:00:52.858.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jens Möller |
SpeciesList | scientific name: Corynebacterium diphtheriae bv. mitis str. ISS 3319; NCBI TaxID: 1450520; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-25 02:57:48 | ID requested | |
⏵ 1 | 2021-03-12 00:00:53 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: diphtheria, host-pathogen interaction, label-free, metabolic pathway, proteomics |
Contact List
Andreas Burkovski |
contact affiliation | Department Biologie Professur für Mikrobiologie (Prof. Dr. Burkovski) |
contact email | andreas.burkovski@fau.de |
lab head | |
Jens Möller |
contact affiliation | FAU Erlangen |
contact email | Jens.moeller@fau.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024381
- Label: PRIDE project
- Name: Corynebacterium diphtheriae proteome adaptation to cell culture medium and serum