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PXD024352

PXD024352 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress
DescriptionThe heat shock response is an ancient and ubiquitous program allowing organisms to survive adverse environmental conditions. In S. cerevisiae, three transcription factors, Hsf1, Msn2 and Msn4, are thought to regulate the stress response. While Msn2/4 can be deleted, Hsf1 is essential. By combining the depletion of Hsf1 with the deletion of Msn2 and Msn4, we were able to switch off the central stress response. We show that the transcription factors Hsf1 and Msn2/4 follow different strategies and regimes: Whereas Msn2/4 are responsible for a broad metabolic response, Hsf1 triggers a direct chaperone response to stabilize and repair unfolded proteins. Exposure of cells lacking Msn2/4 and Hsf1 to thermal stress resulted in massive protein aggregation. Comparison with wildtype yeast revealed that among the proteins rescued by the stress response are many essential proteins.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:28:09.131.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMoritz Mühlhofer
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-24 04:09:02ID requested
12024-01-31 09:31:42announced
22024-10-22 06:28:09announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Hsf1, Msn4, Msn2, Heat shock response
Contact List
Johannes Buchner
contact affiliationTechnische Universität München Lichtenbergstraße 4 85748 Garching Deutschland
contact emailjohannes.buchner@tum.de
lab head
Moritz Mühlhofer
contact affiliationDept. Chemie Lichtenbergstraße 4 85748 Garching
contact emailmoritz.muehlhofer@tum.de
dataset submitter
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Dataset FTP location
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