PXD024245
PXD024245 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Coupling of Cdc20 inhibition and activation by BubR1 |
Description | Tight regulation of the APC/C-Cdc20 ubiquitin ligase that targets Cyclin B1 for degradation is important for mitotic fidelity. The spindle assembly checkpoint (SAC) inhibits Cdc20 through the mitotic checkpoint complex (MCC). In addition, phosphorylation of Cdc20 by Cyclin B1-Cdk1 independently inhibits APC/C-Cdc20 activation. This creates a conundrum for how Cdc20 gets activated prior to Cyclin B1 degradation. Here we show that the MCC component BubR1 harbours both Cdc20 inhibition and activation activities, allowing for cross-talk between the two Cdc20 inhibition pathways. Specifically BubR1 acts as a substrate specifier for PP2A-B56 to enable efficient Cdc20 dephosphorylation in the MCC. A mutant Cdc20 mimicking the dephosphorylated state escapes a mitotic checkpoint arrest arguing that restricting Cdc20 dephosphorylation to the MCC is important. Collectively our work reveals how Cdc20 can be dephosphorylated in the presence of Cyclin B1-Cdk1 activity without causing premature anaphase onset. |
HostingRepository | MassIVE |
AnnounceDate | 2021-03-17 |
AnnouncementXML | Submission_2021-03-17_12:51:10.871.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Mark Adamo |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Carbamidomethyl; Oxidation; Phospho; TMT6plex |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-02-18 14:24:24 | ID requested | |
⏵ 1 | 2021-03-17 12:51:11 | announced |
Publication List
no publication |
Keyword List
submitter keyword: Cdc20, BubR1, APC/C, ubiquitin ligase, Cyclin B1, mitosis, spindle, SAC, MCC, PP2A-B56, Cdk1, dephosphorylation, anaphase |
Contact List
Arminja Kettenbach | |
---|---|
contact affiliation | The Geisel School of Medicine at Dartmouth |
contact email | arminja.n.kettenbach@dartmouth.edu |
lab head | |
Mark Adamo | |
contact affiliation | Dartmouth College |
contact email | mark.e.adamo@dartmouth.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086910/ |