PXD024085

PXD024085 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Streptomyces coelicolor vesicles
Description	Streptomyces coelicolor is a model organism for the study of Streptomyces, a genus of Gram-positive bacteria that undergoes a complex life cycle and produces an enormous repertoire of bioactive metabolites and extracellular enzymes. This study investigated the production and characterization of membrane vesicles (MVs) in liquid cultures of S. coelicolor M145 from a structural and biochemical point of view using microscopic, physical and -omics analyzes. Two main populations of MVs, F3 and F4 MVs, with different size and cargo were isolated and purified. S. coelicolor MV cargo is complex and contains many “messages” such as proteins and metabolites. A total of 166 proteins involved in cell metabolism and differentiation, molecular processing and transport was identified in MVs. In particular, a subset of these proteins was protected from the degradation after proteinase K treatment, indicating their localization inside the vesicles. Vesicle proteome includes many stress response proteins which also play an important role in S. coelicolor morpho- physiological differentiation. Moreover, MVs packaged with an array of metabolites such as antibiotics, vitamins, amino acids and components of carbon metabolism. The analysis of S. coelicolor MV cargo will provide informations to elucidate their biogenesis and functions
HostingRepository	PRIDE
AnnounceDate	2021-11-02
AnnouncementXML	Submission_2021-11-02_07:27:23.598.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giovanni Renzone
SpeciesList	scientific name: Streptomyces coelicolor A3(2); NCBI TaxID: 100226;
ModificationList	2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-09 07:50:14	ID requested
⏵ 1	2021-11-02 07:27:23	announced

Dataset with its publication pending

submitter keyword: Streptomyces coelicolor vesicles, proteomics, LC-MSMS analysis

Anna Maria Puglia
contact affiliation	Laboratory of Molecular Microbiology and Biotechnology, STEBICEF Department, University of Palermo, 90128 Palermo, Italy
contact email	a.maria.puglia@unipa.it
lab head
Giovanni Renzone
contact affiliation	ISPAAM-CNR
contact email	giovanni.renzone@cnr.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024085

PRIDE project URI

• PRIDE
  1. PXD024085
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Streptomyces coelicolor vesicles