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PXD024043

PXD024043 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUltra-high sensitivity mass spectrometry quantifies single-cell proteome changes upon perturbation
DescriptionSingle-cell technologies are revolutionizing biology but are today mainly limited to imaging and deep sequencing. However, proteins are the main drivers of cellular function and in-depth characterization of individual cells by mass spectrometry (MS)-based proteomics would thus be highly valuable and complementary. Here, we develop a robust workflow combining miniaturized sample preparation, very low flow-rate chromatography and a novel trapped ion mobility mass spectrometer, resulting in a more than ten-fold improved sensitivity. We precisely and robustly quantify proteomes and their changes in single, FACS-isolated cells. Arresting cells at defined stages of the cell cycle by drug treatment retrieves expected key regulators. Furthermore, it highlights potential novel ones and allows cell phase prediction. Comparing the variability in more than 430 single-cell proteomes to transcriptome data revealed a stable core proteome despite perturbation, while the transcriptome appears stochastic. Our technology can readily be applied to ultra-high sensitivity analyses of tissue material, posttranslational modifications and small molecule studies from small cell counts to gain unprecedented insights into cellular heterogeneity in health and disease.  
HostingRepositoryPRIDE
AnnounceDate2022-02-23
AnnouncementXMLSubmission_2022-02-23_06:58:01.689.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMario Oroshi
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-08 03:33:59ID requested
12022-02-23 06:58:02announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Proteomics at single-cell resolution
Low flow LC-MS
Drug perturbation
Systems biology
Single-cell heterogeneity
Contact List
Matthias Mann
contact affiliationDepartment of Proteomics and Signal Transduction Max Planck Institute of Biochemistry
contact emailmmann@biochem.mpg.de
lab head
Mario Oroshi
contact affiliationProteomics
contact emailoroshi@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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