PXD024035 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Orphan nuclear receptor COUP-TFII enhances myofibroblasts glycolysis leading to kidney fibrosis |
Description | Recent studies demonstrated that metabolic disturbance, such as augmented glycolysis, contributes to fibrosis. The molecular regulation of this metabolic perturbation in fibrosis, however, has been elusive. COUP-TFII (also known as NR2F2) is an important regulator of glucose and lipid metabolism. Its contribution to organ fibrosis is undefined. Here, we found increased COUP-TFII expression in myofibroblasts in human fibrotic kidneys, lungs, kidney organoids, and mouse kidneys after injury. Genetic ablation of COUP-TFII in mice resulted in attenuation of injury-induced kidney fibrosis. A non-biased proteomic study revealed the suppression of fatty acid oxidation and the enhancement of glycolysis pathways in COUP-TFII overexpressing fibroblasts. Overexpression of COUP-TFII in fibroblasts induced augmented glycolysis and production of alpha smooth muscle actin (αSMA) and collagen1. Knockout of COUP-TFII decreased glycolysis and collagen1 levels in fibroblasts. Chip-qPCR revealed the binding of COUP-TFII on the promoter of PGC1α. Overexpression of COUP-TFII reduced the cellular level of PGC1α. Targeting COUP-TFII serves as a novel treatment approach for mitigating fibrosis in chronic kidney disease and potentially fibrosis in other organs. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-27 |
AnnouncementXML | Submission_2021-04-26_22:13:23.549.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marian Kalocsay |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-07 23:59:29 | ID requested | |
⏵ 1 | 2021-04-26 22:13:23 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Kidney, Fibrosis, COUP-TFII, myofibroblasts |
Contact List
Li Li |
contact affiliation | Renal Division, Brigham and Women’s Hospital, Boston, Massachusetts, USA. Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. |
contact email | lli29@bwh.harvard.edu |
lab head | |
Marian Kalocsay |
contact affiliation | Harvard Medical School |
contact email | marian_kalocsay@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024035
- Label: PRIDE project
- Name: Orphan nuclear receptor COUP-TFII enhances myofibroblasts glycolysis leading to kidney fibrosis