PXD023976

PXD023976 is an original dataset announced via ProteomeXchange.

Title	Unresolved stalled ribosome complexes restrict cycle progression after genotoxic stress
Description	Translation of damaged mRNA can lead to ribosome stalling, thereby producing incomplete proteins toxic to the cell. The mechanism of ribosome-associated quality control (RQC) disassembles stalled ribosomes through the actions of the ASC-1 complex (ASCC). Here, we show that some reagents that chemically damage RNA, such as ultraviolet light (UV), cause ribosome stalling, which leads to accumulation of the ASC-1 complex (ASCC) on stalled ribosomes and stable interaction of the ASCC3 helicase with RNA. In contrast, the ASCC was not similarly affected by emetine or anisomycin-induced ribosome stalling. Our work identified two different types of stalled ribosome. Ribosomes arrested by emetine or anisomycin are transient as they are resolved by the ASCC. Whereas the ASCC fails to split some stalled ribosomes, such as those induced by UV, resulting in long-lived stalled ribosome complexes. We show that ribosome stalling activates the G1/S and G2/M cell cycle checkpoints with long-lived stalled ribosomes causing prolonged checkpoint activation. Thus, the cell adjusts this adaptive survival response to match the nature of the stalled ribosome.
HostingRepository	PRIDE
AnnounceDate	2022-02-07
AnnouncementXML	Submission_2022-02-07_09:01:21.013.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023976
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rebecca Jukes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-03 09:01:37	ID requested
⏵ 1	2022-02-07 09:01:21	announced

Dataset with its publication pending

submitter keyword: Ribosome-associated quality control, RNA interactome capture, RNA damage, ASC-1 complex, ASCC3

Prof. Anne Willis
contact affiliation	MRC Toxicology Unit, University of Cambridge, UK
contact email	aew80@mrc-tox.cam.ac.uk
lab head
Rebecca Jukes
contact affiliation	MRCTox
contact email	rj396@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023976
     1. Label: PRIDE project
     2. Name: Unresolved stalled ribosome complexes restrict cycle progression after genotoxic stress