PXD023873 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | AMPylation profiling during neuronal differentiation reveals extensive variation on lysosomal proteins |
Description | Protein AMPylation is a prevalent posttranslational modification with an emerging role in neurodevelopment and neurodegeneration. Although in metazoans the two highly conserved protein AMP-transferases together with diverse group of AMPylated proteins have been identified using chemical proteomics and biochemical techniques the function of this modification remains largely unknown. Particularly problematic is a localisation of thus far identified AMPylated proteins and putative AMP-transferases. Here, we uncover protein AMPylation as a novel lysosomal protein posttranslational modification characteristic for differentiating neurons. The AMPylated soluble form of exonuclease PLD3 localised in lysosomes shows dramatic increase during the differentiation of neuronal cell lineages. Similar AMPylation pattern has been observed for a lysosomal acid phosphatase ACP2. Our discovery was enabled by combination of chemical proteomics and novel gel-based separation technique of modified and non-modified proteins. Together, our findings expose further the connection between the protein AMPylation and neurodevelopment and reveal a novel lysosomal posttranslational modification. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-27 |
AnnouncementXML | Submission_2021-12-27_08:31:34.986.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tobias Becker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-28 09:03:17 | ID requested | |
1 | 2021-12-01 06:09:37 | announced | |
⏵ 2 | 2021-12-27 08:31:35 | announced | 2021-12-27: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Lysosome,AMPylation |
Contact List
Dr. Pavel Kielkowski |
contact affiliation | LMU Munich, Department of Chemistry |
contact email | pavel.kielkowski@cup.lmu.de |
lab head | |
Tobias Becker |
contact affiliation | Ludwig-Maximilians-University Junior Group Dr. Kielkowski |
contact email | tobias.becker@cup.lmu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023873
- Label: PRIDE project
- Name: AMPylation profiling during neuronal differentiation reveals extensive variation on lysosomal proteins