PXD023811

PXD023811 is an original dataset announced via ProteomeXchange.

Title	Staphylococcus aureus Trigger factor is involved in biofilm formation and cooperates with the chaperone PpiB
Description	Peptidyl-prolyl cis/trans isomerases (PPIases) are enzymes that assist in protein folding around proline-peptide bonds, and often possess chaperone activity. Staphylococcus aureus encodes three PPIases; PrsA, PpiB and Trigger factor (TF). Previous work by our group demonstrated a role for both PrsA and PpiB in S. aureus, however, TF remains largely unstudied. Here, we identify a role for TF in S. aureus biofilm formation, and demonstrate cooperation between TF and the cytoplasmic PPIase PpiB. Mutation of the tig gene (encoding TF) leads to reduced biofilm development in vitro but no significant attenuation of virulence in a mouse model of infection. To determine if TF contributes to biofilm formation via chaperone activity, we analyzed the ability of a tig mutant to survive acid and basic stress. While there was no significant decrease in a tig mutant, a ppiB/tig double mutant exhibited a significant decrease in cell viability after acid and base challenge. We then demonstrate that a ppiB/tig double mutant has exacerbated phenotypes in vitro and in vivo when compared to either single mutant. Finally, in vivo immunoprecipitation of epitope tagged PpiB reveals that PpiB interacts with four times the number of proteins when TF is absent from the cell, suggesting it may be compensating for the loss of TF. Interestingly, the only proteins found to interact with TF are TF itself, FnBPB and the chaperone protein ClpB. Collectively, these results support the first phenotype for S. aureus TF and demonstrate a greater network of cooperation between chaperone proteins in Staphylococcus aureus.
HostingRepository	PRIDE
AnnounceDate	2021-01-28
AnnouncementXML	Submission_2021-01-27_22:11:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ronan Carroll
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-25 23:17:45	ID requested
⏵ 1	2021-01-27 22:11:26	announced

Dataset with its publication pending

submitter keyword: Staphylococcus aureus, Virulence, Trigger factor, FK506 Binding proteins, Cyclophilins

Ronan Carroll
contact affiliation	Department of Biological Sciences, Ohio University
contact email	carrolr3@ohio.edu
lab head
Ronan Carroll
contact affiliation	Ohio University
contact email	carrolr3@ohio.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023811

PRIDE project URI

• PRIDE
  1. PXD023811
     1. Label: PRIDE project
     2. Name: Staphylococcus aureus Trigger factor is involved in biofilm formation and cooperates with the chaperone PpiB