<<< Full experiment listing

PXD023756

PXD023756 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative Proteomic Analysis of Fucosylated Glycoproteins in Bacteroides thetaiotaomicron under Different Polysaccharide Nutrition Conditions
DescriptionBacteroides thetaiotaomicron, one of the most eminent representative gut commensal Bacteroides species, is able to use the L-fucose in host-derived and dietary polysaccharides to modify its capsular polysaccharides and glycoproteins through a mammalian-like salvage metabolic pathway. This process is essential for the colonization of the bacteria and for symbiosis with the host. However, despite the importance of fucosylated proteins (FGPs) in Bacteroide thetaiotaomicron, their types, distribution, and functions remain unclear. In this project, the effects of different saccharide (glucose, corn starch, mucin, and fucoidan) nutrition conditions on FGP expressions and fucosylation are investigated using a chemical biological method based on metabolic labeling and bioorthogonal reaction. According to the results of label-free quantification, 559 FGPs (205 downregulated and 354 upregulated) are affected by the dietary conditions. Of these differentially expressed proteins, 65 proteins show extremely sensitive fucosylation levels. Specifically, the fucosylation of the chondroitin sulfate ABC enzyme, Sus proteins, and cationic efflux system proteins varies significantly upon the addition of mucin, corn starch, or fucoidan. Moreover, these polysaccharides can trigger an appreciable increase in the fucosylation level of the two-component system and ammonium transport proteins. These results highlight the efficiency of the combined metabolic glycan labeling and bio-orthogonal reaction in enriching the intestinal Bacteroide glycoproteins. Moreover, it emphasize the sensitivity of Bacteroides fucosylation to polysaccharide nutrition conditions, which allows for the regulation of bacterial growth.
HostingRepositoryPRIDE
AnnounceDate2022-02-08
AnnouncementXMLSubmission_2022-02-07_17:50:07.965.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHao Jiang
SpeciesList scientific name: Bacteroides thetaiotaomicron VPI-5482; NCBI TaxID: 226186;
ModificationListfucosylated
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-22 06:47:40ID requested
12022-02-07 17:50:08announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Fucosylated glycoprotein,Click chemistry, Bacteroides thetaiotaomicron, Polysaccharide
Contact List
Hao Jiang
contact affiliationOcean University of China
contact emailhaojiang@ouc.edu.cn
lab head
Hao Jiang
contact affiliationSchool of Pharrmacy and Medicine, Ocean University of China
contact emailhaojiang@ouc.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023756
PRIDE project URI
Repository Record List
[ + ]