PXD023754 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | ACE2 independent SARS-CoV-2 infection of a human lung cell line |
Description | In a screen of human upper airway cell lines, we identified the H522 lung adenocarcinoma cells as naturally permissive to SARS-CoV-2 infection despite no evidence of ACE2 or TMPRSS2 expression. Temporally resolved transcriptomic and proteomic profiling of H522 cells revealed consistent alterations in the antiviral host cell response, including marked activation of type-I interferon signaling. Focused chemical screens point to important roles for clathrin-mediated endocytosis and endosomal cathepsins in SARS-CoV-2 infection of H522 cells. The natural permissiveness of H522 cells to SARS-CoV-2 infection despite no requirement for ACE2 implies the utilization of an alternative receptor and viral entry mechanism which may have important implications for SARS-CoV-2 pathogenesis in humans. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:33.604.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Dennis Goldfarb |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-22 06:42:59 | ID requested | |
1 | 2022-03-07 08:42:49 | announced | |
⏵ 2 | 2023-11-14 08:51:34 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Covid-19 |
submitter keyword: SARS-CoV-2, COVID19, H522 |
Contact List
Sebla B. Kutluay |
contact affiliation | Department of Molecular Microbiology, Washington University in St. Louis, St. Louis, MO, USA |
contact email | kutluay@wustl.edu |
lab head | |
Dennis Goldfarb |
contact affiliation | Cell Biology and Physiology Institute for Informatics |
contact email | d.goldfarb@wustl.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD023754 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023754
- Label: PRIDE project
- Name: ACE2 independent SARS-CoV-2 infection of a human lung cell line