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PXD023754

PXD023754 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleACE2 independent SARS-CoV-2 infection of a human lung cell line
DescriptionIn a screen of human upper airway cell lines, we identified the H522 lung adenocarcinoma cells as naturally permissive to SARS-CoV-2 infection despite no evidence of ACE2 or TMPRSS2 expression. Temporally resolved transcriptomic and proteomic profiling of H522 cells revealed consistent alterations in the antiviral host cell response, including marked activation of type-I interferon signaling. Focused chemical screens point to important roles for clathrin-mediated endocytosis and endosomal cathepsins in SARS-CoV-2 infection of H522 cells. The natural permissiveness of H522 cells to SARS-CoV-2 infection despite no requirement for ACE2 implies the utilization of an alternative receptor and viral entry mechanism which may have important implications for SARS-CoV-2 pathogenesis in humans.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:33.604.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDennis Goldfarb
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-22 06:42:59ID requested
12022-03-07 08:42:49announced
22023-11-14 08:51:34announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: SARS-CoV-2, COVID19, H522
Contact List
Sebla B. Kutluay
contact affiliationDepartment of Molecular Microbiology, Washington University in St. Louis, St. Louis, MO, USA
contact emailkutluay@wustl.edu
lab head
Dennis Goldfarb
contact affiliationCell Biology and Physiology Institute for Informatics
contact emaild.goldfarb@wustl.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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