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PXD023712

PXD023712 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe esterase PfeE, Achilles heel in the battle for iron between Pseudomonas aeruginosa and Escherichia coli
DescriptionBacteria access iron, a key nutrient, by producing siderophores or using siderophores produced by other microorganisms. The pathogen Pseudomonas aeruginosa produces two siderophores but is also able to pirate enterobactin (ENT), the siderophore produced by Escherichia coli. ENT-Fe complexes are imported across the outer membranes of P. aeruginosa by the two-outer membrane transporters PfeA and PirA. Iron is released from ENT in the P. aeruginosa periplasm by hydrolysis of ENT by the esterase PfeE. We show here that pfeE gene deletion renders P. aeruginosa unable to grow in the presence of ENT because it is unable to access iron via this siderophore. Two-species co-culture under iron-restricted conditions show that P. aeruginosa strongly represses the growth of E. coli as long it is able to produce its own siderophores. Both strains are present in similar proportions in the culture as long as the siderophore-deficient P. aeruginosa strain is able to use ENT produced by E. coli to access iron. If pfeE is deleted, E. coli has the upper hand in the culture and P. aeruginosa growth is repressed. Overall, these data show that PfeE is the Achilles heel of P. aeruginosa in communities with bacteria producing ENT.
HostingRepositoryPRIDE
AnnounceDate2021-04-29
AnnouncementXMLSubmission_2021-04-29_08:14:40.396.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD023712
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLauriane Kuhn
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-20 09:04:29ID requested
12021-04-29 08:14:40announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: PfeE, Pseudomonas, iron, siderophore, co-culture
Contact List
Isabelle Schalk
contact affiliationUniversité de Strasbourg, InnoVec, UMR7242, ESBS, Bld Sébastien Brant, F-67413 Illkirch, Strasbourg, France. CNRS, UMR7242, ESBS, Bld Sébastien Brant, F-67413 Illkirch, Strasbourg, France
contact emailschalk@unistra.fr
lab head
Lauriane Kuhn
contact affiliationCNRS, IBMC, FRC1589
contact emaill.kuhn@ibmc-cnrs.unistra.fr
dataset submitter
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