PXD023694

PXD023694 is an original dataset announced via ProteomeXchange.

Title	Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoids
Description	Matrigel, a mouse tumor extracellular matrix (ECM) protein mixture, is an indispensable component of most organoid tissue culture. However, it has limited the utility of organoids for drug development and regenerative medicine due to its tumor-derived origin, batch-to batch variation, high cost, and safety issues. Here, we demonstrate that gastrointestinal (GI) tissue-derived ECM hydrogels are a suitable substitute for Matrigel in GI organoid culture. We found that the development and function of GI organoids grown in GI ECM hydrogels are comparable or often superior to those in Matrigel. In addition, GI ECM hydrogels enabled long-term subculture and transplantation of GI organoids by providing GI tissue-mimetic microenvironments. Tissue-specific and age-related ECM profiles of GI ECM hydrogels that affect organoid development were also elucidated through proteomic analysis. Together, our results suggest that ECM hydrogels derived from decellularized GI tissues are an effective alternative to the current gold standard, Matrigel, and produce organoids suitable for GI disease modeling, drug development, and tissue regeneration.
HostingRepository	PRIDE
AnnounceDate	2022-02-10
AnnouncementXML	Submission_2022-02-10_15:12:39.638.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jae Hun Jung
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Sus scrofa; NCBI TaxID: 9823;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-20 01:36:54	ID requested
⏵ 1	2022-02-10 15:12:39	announced

Dataset with its publication pending

submitter keyword: Organoid, Matrigel, LC-MS/MS

Jae Hun Jung
contact affiliation	Yonsei university
contact email	jaehun.jung.88@gmail.com
lab head
Jae Hun Jung
contact affiliation	KHU
contact email	ultramanyo@naver.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023694
     1. Label: PRIDE project
     2. Name: Tissue extracellular matrix hydrogels as alternative to Matrigel for culturing gastrointestinal organoids