PXD023542 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context |
Description | Discovery of host-pathogen intra-cellular interactions is critical for identification of mechanisms of infection and discovery. Affinity purification based on Mass Spectrometry (AP-MS) approaches can identify the host-pathogen protein-protein interaction network that includes hundreds of cellular proteins. For example, over 330 interactions were identified for SARS-CoV-2 major proteins. However, identification of the critical anchoring interactions directly induced by the viral proteins is still challenging. Here we developed a complete workflow of in-situ XL-MS followed by AP-MS, and employ it to investigate SARS-CoV2 protein inside the host cell. We focused on three Sars-Cov-2 proteins for which the structural information is either missing or incomplete: NSP1, NSP2, and the Nucleocapsid protein (N protein). AP-MS revealed that several human proteins were co-eluting with NSP1 and NSP2 while protein N eluted alone. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:51:56.842.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Moriya Slavin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-12 03:15:29 | ID requested | |
1 | 2022-02-19 11:08:52 | announced | |
⏵ 2 | 2023-11-14 08:51:57 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Covid-19 |
submitter keyword: AP-MS, Mass-Spectrometry, SARS-CoV-2, HEK293, Nucleocapsid protein,LC-MSMS, Nsp1, Strep-tag, Nsp2 |
Contact List
Nir Kalisman |
contact affiliation | Dept. of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel |
contact email | nirka@mail.huji.ac.il |
lab head | |
Moriya Slavin |
contact affiliation | Biochemistry department in the Hebrew University |
contact email | moriya.slavin@mail.huji.ac.il |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023542
- Label: PRIDE project
- Name: Targeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context