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PXD023542

PXD023542 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted in situ cross-linking for determination of structure and interactions of SARS-CoV-2 proteins in the cellular context
DescriptionDiscovery of host-pathogen intra-cellular interactions is critical for identification of mechanisms of infection and discovery. Affinity purification based on Mass Spectrometry (AP-MS) approaches can identify the host-pathogen protein-protein interaction network that includes hundreds of cellular proteins. For example, over 330 interactions were identified for SARS-CoV-2 major proteins. However, identification of the critical anchoring interactions directly induced by the viral proteins is still challenging. Here we developed a complete workflow of in-situ XL-MS followed by AP-MS, and employ it to investigate SARS-CoV2 protein inside the host cell. We focused on three Sars-Cov-2 proteins for which the structural information is either missing or incomplete: NSP1, NSP2, and the Nucleocapsid protein (N protein). AP-MS revealed that several human proteins were co-eluting with NSP1 and NSP2 while protein N eluted alone.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:51:56.842.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMoriya Slavin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-12 03:15:29ID requested
12022-02-19 11:08:52announced
22023-11-14 08:51:57announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: AP-MS, Mass-Spectrometry, SARS-CoV-2, HEK293, Nucleocapsid protein,LC-MSMS, Nsp1, Strep-tag, Nsp2
Contact List
Nir Kalisman
contact affiliationDept. of Biological Chemistry, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel
contact emailnirka@mail.huji.ac.il
lab head
Moriya Slavin
contact affiliationBiochemistry department in the Hebrew University
contact emailmoriya.slavin@mail.huji.ac.il
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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