PXD023431

PXD023431 is an original dataset announced via ProteomeXchange.

Title	Secretomic analysis of a novel strain SM1988
Description	Strain SM1988T is a Gram-negative, aerobic, oxidase- and catalase-positive, unipolar flagellated, and rod-shaped bacterium capable of hydrolyzing casein, gelatin and collagens. Phylogenetic analysis revealed that strain SM1988T formed a distinct phylogenetic lineage along with known genera within the family Pseudoalteromonadaceae, with 16S rRNA gene sequence similarity being less than 93.3% to all known species in the family. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data, strain SM1988T was considered to represent a novel species in a novel genus in the family Pseudoalteromonadaceae, for which the name Flocculibacter collagenilyticus gen. nov., sp. nov. is proposed, with the type strain being SM1988T (= MCCC 1K04279T = KCTC 72761T). Strain SM1988T showed a high production (236 U/mL) of extracellular collagenases, which had high activity against both bovine collagen and codfish collagen. Biochemical tests combined with genomic and secretomic analyses indicated that the collagenases secreted by strain SM1988T are serine proteases from the S8 family. These data suggest that strain SM1988T acts as an important player in marine collagen degradation and recycling and may have a promising potential in collagen resource utilization.
HostingRepository	PRIDE
AnnounceDate	2021-01-14
AnnouncementXML	Submission_2021-01-14_00:01:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Li
SpeciesList	scientific name: Pseudoalteromonas sp. s-12; NCBI TaxID: 1089176;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-06 01:45:34	ID requested
⏵ 1	2021-01-14 00:01:29	announced

Dataset with its publication pending

submitter keyword: Flocculibacter gen. nov., collagenase, collagenase-secreting bacteria, the S8 family proteases, secretome analysis

Yuzhong Zhang
contact affiliation	Institute of Microbiology, Shandong University, China
contact email	zhangyz@sdu.edu.cn
lab head
Jian Li
contact affiliation	Shandong University
contact email	fighter1216217564@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD023431

PRIDE project URI

• PRIDE
  1. PXD023431
     1. Label: PRIDE project
     2. Name: Secretomic analysis of a novel strain SM1988