PXD023361 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Cdk8 kinase module regulates Mediator-RNA polymerase II interaction |
Description | We use biochemically reconstituted transcription initiation components including the Mediator core and Cdk8 kinase modules (CKM) to investigate the function of the CKM in regulating the Mediator-RNA polymerase II interaction in a highly purified system. We use cross-linking coupled to mass spectrometry to map the interaction of the CKM and core Mediator, and in vitro phosphorylation assays followed by phosphopeptide enrichment coupled to mass spectrometry to identify Cdk8 phosphorylation targets in this context and biochemically dissect their functions. Finally, we investigate the function of phosphorylation by Cdk8 on transcription in vivo. Based on that, we propose a model to integrate the phosphorylation-dependent and -independent functions of the CKM in transcription initiation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-28 |
AnnouncementXML | Submission_2021-04-28_10:27:03.750.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Fanni Laura Bazso |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-04 01:48:22 | ID requested | |
⏵ 1 | 2021-04-28 10:27:04 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNA polymerase II, transcription, Mediator, Cdk8 |
Contact List
Henning Urlaub |
contact affiliation | Max Planck Institute for Biophysical Chemistry, Göttingen,Germany and Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany |
contact email | henning.urlaub@mpibpc.mpg.de |
lab head | |
Fanni Laura Bazso |
contact affiliation | Max Planck Institute for Biophysical Chemistry |
contact email | fanni-laura.bazso@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD023361 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023361
- Label: PRIDE project
- Name: The Cdk8 kinase module regulates Mediator-RNA polymerase II interaction