<<< Full experiment listing

PXD023255

PXD023255 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGenetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury
DescriptionRIP1 kinase-mediated inflammatory and cell death pathways have been implicated in the pathology of acute and chronic disorders of the nervous system. Here, we describe a novel animal model of RIP1 kinase deficiency, generated by knock-in of the kinase-inactivating RIP1(D138N) mutation in rats. Homozygous RIP1 kinase-dead (KD) rats had normal development, reproduction and did not show any gross phenotypes at baseline. However, cells derived from RIP1 KD rats displayed resistance to necroptotic cell death. In addition, RIP1 KD rats were resistant to TNF-induced systemic shock. We studied the utility of RIP1 KD rats for neurological disorders by testing the efficacy of the genetic inactivation in the transient middle cerebral artery occlusion/reperfusion model of brain injury. RIP1 KD rats were protected in this model in a battery of behavioral, imaging and histopathological endpoints. In addition, RIP1 KD rats had reduced inflammation and accumulation of neuronal injury biomarkers. Unbiased proteomics in the plasma identified additional changes that were ameliorated by RIP1 genetic inactivation. Together these data highlight the utility of the RIP1 KD rats for target validation and biomarker studies for neurological disorders. For mass spectrometry experiment, please check the method section in the manuscript.
HostingRepositoryMassIVE
AnnounceDate2021-04-01
AnnouncementXMLSubmission_2021-04-01_11:17:27.610.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMeena Choi
SpeciesList scientific name: Sprague Dawley;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-21 09:05:53ID requested
12021-04-01 11:17:27announced
Publication List
no publication
Keyword List
submitter keyword: DIA, Thermo Fusion Lumos, HRM, rat plasma, RIP1 kinase, ischemic brain injury
Contact List
Baris Bingol
contact affiliationGenentech, Inc.
contact emailbarisb@gene.com
lab head
Meena Choi
contact affiliationNortheastern University
contact emailmnchoi67@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086623/