PXD023218 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Linking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling |
Description | Proteome-wide measurements of protein turnover have largely ignored the impact of post-translational modifications (PTMs). To address this gap, we employ stable isotope labelling and mass spectrometry to measure the turnover of >120,000 peptidoforms including >33,000 phosphorylated, acetylated and ubiquitinated peptides for >9,000 native proteins. This site-resolved protein turnover (SPOT) profiling discloses global and site-specific differences in turnover associated with the presence or absence of PTMs. While causal relationships may not always be immediately apparent, we hypothesize that PTMs with diverging turnover may distinguish states of differential protein stability, structure, localization, enzymatic activity, or protein-protein interactions. We exemplify how the turnover data may facilitate insights into unknown functions of PTMs and provide a web-tool for the scientific community that allows interrogation and visualisation of all turnover data. Since the methodology is applicable to many cell types and modifications, it has substantial potential to prioritize PTMs for functional investigation in the future. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-10 |
AnnouncementXML | Submission_2021-11-10_13:25:54.375.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jana Zecha |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitinylated lysine; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-18 01:48:40 | ID requested | |
⏵ 1 | 2021-11-10 13:25:54 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Human, Turnover, PTMs, SILAC |
Contact List
Bernhard Kuster |
contact affiliation | Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany |
contact email | kuster@tum.de |
lab head | |
Jana Zecha |
contact affiliation | TUM |
contact email | jana.zecha@tum.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023218
- Label: PRIDE project
- Name: Linking post-translational modifications and protein turnover by site-resolved protein turnover (SPOT) profiling