PXD023109

PXD023109 is an original dataset announced via ProteomeXchange.

Title	Protein-based vaccine protect against Piscirickettsia salmonis in Atlantic salmon (Salmo salar)
Description	An effective and economical vaccine against the Piscirickettsia salmonis pathogen is needed for sustainable salmon farming and to reduce disease-related economic losses. Consequently, the aquaculture industry urgently needs to investigate efficient prophylactic measures. Three protein-based vaccine prototypes against Piscirickettsia salmonis were prepared from a highly pathogenic Chilean isolate. Only one vaccine effectively protected Atlantic salmon (Salmo salar), in correlation with the induction of Piscirickettsia-specific IgM antibodies and a high induction of transcripts encoding pro-inflammatory cytokines (i.e. Il-1β and TNF-α). In addition, we studied the proteome fraction protein of P. salmonis strain Austral-005 using multidimensional protein identification technology. The analyzes identified 87 proteins of different subcellular origins, such as the cytoplasmic and membrane compartment, where many of them have virulence functions. The other two prototypes activated only the innate immune responses, but did not protect Salmo salar against Piscirickettsia salmonis. These results suggest that the knowledge of the formulation of vaccines based on P. salmonis proteins is useful as an effective therapy, this demonstrates the importance of the different research tools to improve the study of the different immune responses, resistance to diseases in the Atlantic salmon. We suggest that this vaccine can help prevent widespread infection by P. salmonis, in addition to being able to be used as a booster after a primary vaccine to maintain high levels of circulating protective antibodies, greatly helping to reduce the economic losses caused by the pathogen.
HostingRepository	PRIDE
AnnounceDate	2021-02-16
AnnouncementXML	Submission_2021-02-15_22:39:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mauricio Hernandez
SpeciesList	scientific name: Piscirickettsia salmonis AUSTRAL-005; NCBI TaxID: 1398558;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ FT; LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-14 01:11:08	ID requested
⏵ 1	2021-02-15 22:39:41	announced

Dataset with its publication pending

submitter keyword: Piscirickettsia salmonis, Atlantic salmon, adaptive, innate, vaccine, IL-1β, Proteome, IgM.

Mauricio Hernandez
contact affiliation	proteogenomics laboratory
contact email	mhernandez@melisainstitute.org
lab head
Mauricio Hernandez
contact affiliation	Proteogenomics laboratory, MELISA Institute
contact email	mauricio.hernandez@uach.cl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023109
     1. Label: PRIDE project
     2. Name: Protein-based vaccine protect against Piscirickettsia salmonis in Atlantic salmon (Salmo salar)