PXD023048

PXD023048 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based proteomic analysis of dentate gyrus in temporal lobe epilepsy with hippocampal sclerosis
Description	Temporal lobe epilepsy (TLE) is the most frequent type of focal epilepsy in adults, typically resistant to pharmacological treatment and mostly present with cognitive impairment and psychiatric comorbidities. The most common neuropathological hallmark in TLE patients is hippocampal sclerosis(HS). However, the underlying molecular mechanisms involved remain poorly characterized. Dentate gyrus(DG), one specific hippocampal subarea, structural and functional changes imply a key involvement of the DG in the development of TLE. In this study, isobaric tags for relative and absolute quantitation (iTRAQ) -based quantitative proteomic technique was performed to analysis of hippocampal DG obtained from patients with TLE-HS compared to control samples obtained from the autopsy. Our proteomic data identified 5583 proteins, of which 82 proteins were up-regulated and 90 proteins were down-regulated. Bioinformatics analysis indicated that differential expressed proteins enriched in “synaptic vesicle”, “mitochondrion”, “cell-cell adhesion”, “regulation of synaptic plasticity”, “ATP binding” and “Oxidative phosphorylation”. Protein-protein interaction network analysis found a pivotal module of 10 proteins that relate to “Oxidative phosphorylation”. This study is the first to investigate proteomic alterations in DG region of TLE-HS patients, and pave the way to better understanding of epileptogenesis mechanisms and future therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2021-01-18
AnnouncementXML	Submission_2021-01-17_23:16:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongyu Long
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-10 06:36:28	ID requested
⏵ 1	2021-01-17 23:16:44	announced

Dataset with its publication pending

submitter keyword: temporal lobe epilepsy, hippocampal sclerosis, dentate gyrus, quantitative proteomics, iTRAQ

Hongyu Long
contact affiliation	Department of Neurology, Xiangya Hospital, Central South University
contact email	longhongyu@csu.edu.cn
lab head
Hongyu Long
contact affiliation	Department of Neurology, Xiangya Hospital, Central South University
contact email	longhongyu@csu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023048
     1. Label: PRIDE project
     2. Name: iTRAQ-based proteomic analysis of dentate gyrus in temporal lobe epilepsy with hippocampal sclerosis