PXD023047

PXD023047 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis EARLY RESPONSIVE TO DEHYDRATION 7 associates with lipid droplets via its senescence domain
Description	Lipid droplets (LDs) are neutral lipid-containing organelles found in all kingdoms of life and are decorated with proteins that carry out a variety of functions. Despite the similar subcellular location, the LD proteins that carry out these functions vary between kingdoms, and only a few LD proteins share obvious amino acid sequence identity between plants and mammals. To better understand the many cellular roles of LDs in plants, a more comprehensive inventory and characterization of LD proteins is required. To this end, we analyzed proteomics data of isolated LDs and identified EARLY RESPONSIVE TO DEHYDRATION 7 (ERD7) as a putative LD protein. mCherry-tagged ERD7 localized to both LDs and to the cytosol when transiently expressed in Nicotiana benthamiana leaves, whereas a fragment of ERD7 containing a “senescence domain” (SD) localized exclusively to LDs with no appreciable accumulation in the cytosol. Similarly, the SDs of the two Arabidopsis ERD7 homologs, here termed SENESCENCE-ASSOCIATED A2 (SENA2) and SENA3, localized exclusively to LDs as did the SD of a phylogenetically distant SD-containing protein, human spartin. In contrast, SDs of the Arabidopsis SENESCENCE-ASSOCIATED B (SENB) family proteins did not localize to LDs and instead localized to mitochondria. Arabidopsis erd7 mutants did not display any observable LD phenotypes in seeds, seedlings or senescent leaves, perhaps due to genetic redundancy of the SENA protein family. A yeast-two-hybrid screen revealed that the S4 family of MYB transcription factors interacts with ERD7, providing a potential avenue for the exploration of ERD7 function. In summary, we here report a previously unappreciated family of plant LD-associated proteins, SENA, which contain SDs that localize to LDs.
HostingRepository	PRIDE
AnnounceDate	2021-03-29
AnnouncementXML	Submission_2021-03-29_07:35:58.219.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Till Ischebeck
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-10 06:36:25	ID requested
⏵ 1	2021-03-29 07:35:58	announced

Dataset with its publication pending

submitter keyword: Lipid droplet, drought stress, Arabidopsis, leaves

Till Ischebeck
contact affiliation	Department of Plant Biochemistry, University of Göttingen
contact email	tischeb@gwdg.de
lab head
Till Ischebeck
contact affiliation	University of Goettingen
contact email	tischeb@gwdg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023047
     1. Label: PRIDE project
     2. Name: Arabidopsis EARLY RESPONSIVE TO DEHYDRATION 7 associates with lipid droplets via its senescence domain