PXD023011

PXD023011 is an original dataset announced via ProteomeXchange.

Title	Lysine lactylation critically regulated energy metabolism of the protozoan parasite Trypanosoma brucei
Description	Lysine lactylation, a new post-translational modification identified on histones of human and mouse cells, stimulates gene transcription from chromatin directly. However, very little is known in the scope and cellular distribution of lactylated proteins. In the present study, we conducted the first proteome-wide survey of lactylated sites in Trypanosoma brucei, a unicellular parasite causing human African sleeping sickness and livestock nagana disease, using LC-MS/MS to identify peptides enriched by immuno-purification with an anti-lactyllysine antibody. Overall, we identified 387 unique lysine lactylated sites in 257 lactylated proteins with diverse cellular localizations and biological functions. Lactylated proteins are involved in a wide variety of cellular functions such as metabolism and gene regulation. Further, we demonstrate that the lactate-derived lactylation in trypanosome is regulated by glucose metabolism. Collectively, our findings provide the first comprehensive view of the lactylome of T. brucei and suggest that lysine lactylation in trypanosomes involves a diverse array of cellular functions.
HostingRepository	PRIDE
AnnounceDate	2021-10-01
AnnouncementXML	Submission_2021-10-01_10:49:22.883.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Naiwen Zhang
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-09 01:34:56	ID requested
⏵ 1	2021-10-01 10:49:23	announced

Dataset with its publication pending

submitter keyword: Lysine lactylation

Trypanosoma brucei

energy metabolism

glycolysis

Qijun Chen
contact affiliation	Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China. 2 The Research Unit for Pathogenic Mechanisms of Zoonotic Parasites, Chinese Academy of Medical Sciences, 120 Dongling Road, Shenyang 110866, China.
contact email	qijunchen759@syau.edu.cn
lab head
Naiwen Zhang
contact affiliation	Shenyang Agricultural University
contact email	naiwenzhang@stu.syau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023011
     1. Label: PRIDE project
     2. Name: Lysine lactylation critically regulated energy metabolism of the protozoan parasite Trypanosoma brucei