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PXD022932

PXD022932 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleImpaired phosphatidylethanolamine metabolism activates a reversible stress response that detects and resolves mutant mitochondrial precursors
DescriptionFor decades, the endoplasmic reticulum (ER)-resident tri-methylation pathway of phosphatidylcholine production that is fed by phosphatidylethanolamine (PE) made in the mitochondrion, served as a phospholipid trafficking paradigm. Recently, the strict mitochondrial localization of the enzyme that makes PE in the mitochondrion, phosphatidylserine decarboxylase 1 (Psd1), was questioned. Since a dual localization of Psd1 to the ER would have far-reaching implications, we initiated our study to independently re-assess the subcellular distribution of Psd1. Our results support the unavoidable conclusion that the vast majority, if not all, of functional Psd1 resides in the mitochondrion. Through our efforts, we discovered that mutant forms of Psd1 that impair a self-processing step needed for it to become functional, are dually localized to the ER when expressed in a PE-limiting environment. We conclude that severely impaired cellular PE metabolism provokes an ER-assisted adaptive response that is capable of identifying and resolving nonfunctional mitochondrial precursors.
HostingRepositoryMassIVE
AnnounceDate2021-02-01
AnnouncementXMLSubmission_2021-02-01_08:11:51.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathan Trinidad
SpeciesList scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-04 08:36:39ID requested
12021-02-01 08:11:52announced
Publication List
no publication
Keyword List
submitter keyword: endoplasmic reticulum phosphatidylethanolamine mitochondria Psd1 metabolism self-processing
Contact List
Steven M. Claypool
contact affiliationJohns Hopkins University School of Medicine
contact emailsclaypo1@jhmi.edu
lab head
Jonathan Trinidad
contact affiliationIndiana University
contact emailtrinidad@indiana.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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