<<< Full experiment listing

PXD022828

PXD022828 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe neomycin resistance cassette in the targeted allele of Shank3B knock-out mice has potential off-target effects to produce an unusual Shank3 isoform
DescriptionVariants of the SH3 and multiple ankyrin repeat domains 3 (SHANK3), which encodes postsynaptic scaffolds, are associated with brain disorders. The targeted alleles in a few Shank3 knock-out (KO) lines contain a neomycin resistance (Neo) cassette, which may perturb the normal expression of neighboring genes; however, this has not been investigated in detail. We previously reported an unexpected increase in the mRNA expression of Shank3 exons 1~12 in the brains of Shank3B KO mice generated by replacing Shank3 exons 13~16 with the Neo cassette. In this study, we confirmed that the increased Shank3 mRNA in Shank3B KO brains produced an unusual ~60 kDa Shank3 isoform (Shank3-N), which did not properly localize to the synaptic compartment.
HostingRepositoryMassIVE
AnnounceDate2021-01-07
AnnouncementXMLSubmission_2021-01-07_17:03:18.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHeeyoun Hwang
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListL-methionine (R)-sulfoxide; iodoacetamide - site C
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-30 23:48:41ID requested
12021-01-07 17:03:18announced
Publication List
no publication
Keyword List
submitter keyword: shank3, neomycin
Contact List
Jin Young Kim
contact affiliationKorea Basic Science Institute
contact emailjinyoung@kbsi.re.kr
lab head
Heeyoun Hwang
contact affiliationKBSI
contact emailheeyounh@kbsi.re.kr
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086539/