PXD022711 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide analysis of heat-stress in Pinus radiata somatic embryos reveals a combined response of sugar metabolism and translational regulation mechanisms |
Description | Recent research has demonstrated that high temperatures during initial stages of conifer somatic embryogenesis can modulate the subsequent phases of the process, as well as the behaviour of the resulting plants ex vitro. In this sense, the development of high-throughput techniques has facilitated the study of the molecular response of plants to numerous stress factors. Proteomics offers a reliable image of the cell status and is known to be extremely susceptible to environmental temperature changes. As a result, in this study, the proteome of radiata pine somatic embryos was studies by LC-MS after the application of high temperatures during induction of embryonal masses (23 °C, 8 weeks, control, 40 °C, 4 h, 60 °C, 5 min). Then, the content of specific soluble sugars and sugar alcohols by HPLC was analysed. Results confirmed a decreased initiation rate of embryonal masses under high temperatures and a slight increase in the production of somatic embryos, as observed in previous studies. Besides, heat provoked a long-term readjustment of the protein synthesis machinery, by regulating translation and the composition of ribosomes. The levels of proteins related to methylation and posttranscriptional regulation mechanisms were also altered under high temperatures, coupled with higher contents of heat shock proteins and chaperones, transmembrane transport proteins, enzymes involved in the synthesis of fatty acids, specific compatible sugars (myo-inositol), and changes in the composition of cell-wall carbohydrates. These modifications occurred at the expense of the synthesis of molecules involved in oxidative stress responses, such as peroxidases, thiamine, phenolic compounds and some amino acids, by a decreased activity of the glycolytic pathway and reduced nitrogen assimilation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-04-09 |
AnnouncementXML | Submission_2021-04-09_05:36:06.759.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Vera Mendes |
SpeciesList | scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347; |
ModificationList | S-carboxamidoethyl-L-cysteine |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-24 09:04:36 | ID requested | |
⏵ 1 | 2021-04-09 05:36:07 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: carbohydrates, compatible solutes, heat shock proteins, high temperatures, methylation, proteomics, radiata pine, somatic embryogenesis. |
Contact List
Paloma Moncaleán |
contact affiliation | Department of Forestry Science, NEIKER, Arkaute, Spain. |
contact email | pmoncalean@neiker.eus |
lab head | |
Vera Mendes |
contact affiliation | Center for Neuroscience and Cell Biology, University of Coimbra |
contact email | vera3m@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD022711 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022711
- Label: PRIDE project
- Name: Proteome-wide analysis of heat-stress in Pinus radiata somatic embryos reveals a combined response of sugar metabolism and translational regulation mechanisms