PXD022576

PXD022576 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics provides insights into diapause program of Bactrocera minax (Diptera: Tephritidae)
Description	The Chinese citrus fly, Bactrocera minax, is a notorious univoltine pest that causes damage to citrus. B. minax enters obligatory pupal diapause in each generation to resist harsh environmental conditions in winter. Despite the enormous efforts that have been made in the past decade, the understanding of pupal diapause of B. minax is currently still fragmentary. In this study, the 20-hydroxyecdysone solution and ethanol solvent was injected into newly-formed pupae to obtain non-diapause- (ND) and diapause-destined (D) pupae, respectively, and a comparative proteomics analysis between ND and D pupae was performed 1 and 15 d after injection. A total of 3,255 proteins were identified, of which 190 and 463 were found to be differentially abundant proteins (DAPs) in ND1 vs D1 and ND15 vs D15 comparisons, respectively. The reliability and accuracy of LFQ method was validated by qRT-PCR. Functional analyses of DAPs, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network construction, were conducted. The results revealed that the diapause program of B. minax is closely associated with several physiological activities, such as phosphorylation, chitin biosynthesis, autophagy, signaling pathways, endocytosis, skeletal muscle formation, protein metabolism, and core metabolic pathways of carbohydrate, amino acid, and lipid conversion. The findings of this study provide insights into diapause program of B. minax and lay a basis for further investigation into its underlying molecular mechanisms.
HostingRepository	PRIDE
AnnounceDate	2020-12-15
AnnouncementXML	Submission_2020-12-14_23:22:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia Wang
SpeciesList	scientific name: Bactrocera minax; NCBI TaxID: 104690;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 01:54:02	ID requested
⏵ 1	2020-12-14 23:22:05	announced

Dataset with its publication pending

submitter keyword: Bactroceraf minax

Jia Wang
contact affiliation	College of plant protection, Southwest University, China
contact email	aimarjia@126.com
lab head
Jia Wang
contact affiliation	College of plant protection, Southwest University
contact email	aimarjia@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/12/PXD022576

PRIDE project URI

• PRIDE
  1. PXD022576
     1. Label: PRIDE project
     2. Name: Comparative proteomics provides insights into diapause program of Bactrocera minax (Diptera: Tephritidae)