PXD022549

PXD022549 is an original dataset announced via ProteomeXchange.

Title	Multiparametric characterization of red blood cells physiology after hypotonic dialysis based drug encapsulation process.
Description	Red blood cells (RBC) can act as carriers for therapeutic agents and given their biocompatibility and long lifespan in the circulation, they can substantially improve the safety, pharmacokinetics, and pharmacodynamics of many drugs. Maintaining RBC integrity and lifespan is important for the efficacy of RBC as drug carrier and can be a complex challenge of drug encapsulation. We investigated the impact of drug encapsulation by hypotonic dialysis on RBC physiology and integrity. Several parameters were compared between processed RBC loaded with L-asparaginase (“eryaspase�), processed RBC without drug and non-processed RBC. Processed RBC were less hydrated and displayed a reduction of intracellular content, relative to non-processed RBC. This reduction of intracellular content changed the RBC metabolomic profile, as indicated by the activation of the pentose phosphate pathway, but no impact on the global RBC proteomic profile was observed. We found that the encapsulation process caused moderate morphological changes and was accompanied by an increase of microparticles. Despite a decrease in their deformability, processed RBC were not mechanically retained in a spleen-mimicking device. Moreover, processed RBC had increased surface-to-volume ratio and osmotic resistance. Finally, the half-life of processed RBC was not significantly affected in a mouse model and our previous phase 1 clinical study showed that encapsulation of asparaginase in RBC prolonged its in vivo half-life compared to free form. Overall, our study demonstrated that encapsulation by hypotonic dialysis may affect certain  several characteristics of RBC, but does not significantly impact on the in vivo longevity of RBC or their drug carrier function.
HostingRepository	PRIDE
AnnounceDate	2021-11-29
AnnouncementXML	Submission_2021-11-29_07:01:49.123.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	François GUILLONNEAU
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-16 03:38:59	ID requested
⏵ 1	2021-11-29 07:01:49	announced

Dataset with its publication pending

submitter keyword: human red blood cells, hypotonic dialysis, drug carriers, characterization, orbitrap fusion, hemoglobin-based absolute and global quantification

Agnes Cibiel
contact affiliation	Erytech 60, avenue Rockefeller • Bâtiment Adénine • 69008 Lyon • France
contact email	agnes.cibiel@erytech.com
lab head
François GUILLONNEAU
contact affiliation	3P5 Université de Paris
contact email	francois.guillonneau@parisdescartes.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD022549

PRIDE project URI

• PRIDE
  1. PXD022549
     1. Label: PRIDE project
     2. Name: Multiparametric characterization of red blood cells physiology after hypotonic dialysis based drug encapsulation process.