PXD022432 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nucleolin rescues TDP-43 toxicity in yeast and human cell models |
Description | TDP-43 is a nuclear protein involved in pivotal processes, extensively studied for its implication in neurodegenerative disorders. TDP-43 cytosolic inclusions are a common neuropathologic hallmark in amyotrophic lateral sclerosis (ALS) and related diseases, and it is now established that TDP-43 misfolding and aggregation play a key role in their etiopathology. TDP-43 neurotoxic mechanisms are not yet clarified, but the identification of proteins able to modulate TDP-43-mediated damage may be promising therapeutic targets for TDP-43 proteinopathies. Here we show by the use of refined yeast models that the nucleolar protein nucleolin (NCL) acts as a potent suppressor of TDP-43 toxicity, restoring cell viability. We provide evidence that NCL co-expression is able to alleviate TDP-43-induced damage also in human cells, further supporting its beneficial effects in a more consistent pathophysiological context. Presented data suggest that NCL could promote TDP-43 nuclear retention, reducing the formation of toxic cytosolic TDP-43 aggregates. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-26 |
AnnouncementXML | Submission_2021-03-25_23:10:52.978.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Roberto Stella |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-09 03:24:50 | ID requested | |
⏵ 1 | 2021-03-25 23:10:53 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Neurodegenerative disorders, TDP-43 proteinopathies, ALS, FTD, nucleolin, nucleo-cytoplasmic transport, TMT, LC-HRMS/MS |
Contact List
Alessandro Bertoli |
contact affiliation | Department of Biomedical Sciences, University of Padova, Italy CNR-Neuroscience Institute, Italy Padova Neuroscience center, University of Padova, Italy |
contact email | alessandro.bertoli@unipd.it |
lab head | |
Roberto Stella |
contact affiliation | Istituto Zooprofilattico Sperimentale delle Venezie |
contact email | rstella@izsvenezie.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022432 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022432
- Label: PRIDE project
- Name: Nucleolin rescues TDP-43 toxicity in yeast and human cell models