PXD022408 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cryo-EM structural analysis of FADD: Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate |
Description | Uncovering the molecular architecture of the core FADD:Caspase-8 complex and how this is altered by regulatory partners, such as the cell death inhibitor c-FLIP, is essential to understand co-ordination of cell fate. Here, using electron microscopy, we visualize for the first time fulllength procaspase-8 in a complex with FADD. Our structural analysis reveals how the FADDnucleated tandem death effector domain (tDED) helical filament is required to correctly orientate procaspase-8 catalytic domains, enabling activation via anti-parallel dimerization. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-08 |
AnnouncementXML | Submission_2021-02-08_00:59:01.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD022408 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rebekah Jukes-Jones |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-06 07:03:52 | ID requested | |
⏵ 1 | 2021-02-08 00:59:01 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cell death. DISC, FADD, Caspase 8, c-Flip Complex, EM, Structure |
Contact List
Marion M. MacFarlane |
contact affiliation | MRC Toxicology Unit, University of Cambridge, Hodgkin Building, Lancaster Road, Leicester, LE1 9HN, UK |
contact email | mm2312@mrc-tox.cam.ac.uk |
lab head | |
Rebekah Jukes-Jones |
contact affiliation | MRC Toxicology Unit |
contact email | rj396@mrc-tox.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022408
- Label: PRIDE project
- Name: Cryo-EM structural analysis of FADD: Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate