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PXD022397

PXD022397 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTbSAP is a novel chromatin protein repressing metacyclic Variant Surface Glycoprotein expression sites in bloodstream form Trypanosoma brucei
DescriptionThe African trypanosome Trypanosoma brucei is a unicellular eukaryote, which relies on a protective Variant Surface Glycoprotein (VSG) coat for survival in the mammalian host. A single trypanosome has >2000 VSG genes and pseudogenes of which only one is expressed from one of ~15 telomeric bloodstream form expression sites (BESs). Infectious metacyclic trypanosomes present within the tsetse fly vector also express VSG from a separate set of telomeric metacyclic ESs (MESs). All MESs are silenced in bloodstream form T. brucei. As very little is known about how this is mediated, we performed a whole genome RNAi library screen to identify MES repressors. This allowed us to identify a novel SAP domain containing DNA binding protein which we called TbSAP. TbSAP is enriched at the nuclear periphery and binds both MESs and BESs. Knockdown of TbSAP in bloodstream form trypanosomes did not result in cells becoming more ‘metacyclic’-like. Instead, there was extensive global upregulation of transcripts including MES VSGs, VSGs within the silent VSG arrays as well as genes immediately downstream of BES promoters. TbSAP therefore appears to be a novel architectural chromatin protein playing an important role in silencing the extensive VSG repertoire of bloodstream form T. brucei.
HostingRepositoryPRIDE
AnnounceDate2020-11-06
AnnouncementXMLSubmission_2020-11-06_06:09:02.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterF Butter
SpeciesList scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-06 01:39:01ID requested
12020-11-06 06:09:02announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC/MS-MS
Contact List
Falk Butter
contact affiliationInstitute of Molecular Biology (IMB)
contact emailf.butter@imb.de
lab head
F Butter
contact affiliationQuantitative Proteomics Institute of Molecular Biology (IMB)
contact emailf.butter@imb-mainz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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