PXD022245

PXD022245 is an original dataset announced via ProteomeXchange.

Title	PTENε, a new isoform of PTEN, suppresses tumor metastasis through filopodia formation regulation.
Description	PTEN is frequently mutated in a wide range of malignancies. Beyond suppressing tumorigenesis, PTEN is involved in multiple biological processes, and the complexity of PTEN function is partially attributed to PTEN family members including PTENα and PTENβ. Here, we report the identification of PTENε (also named as PTEN5), a novel N-terminal-extended PTEN isoform that suppresses tumor invasion and metastasis. We show that the translation of PTENε is initiated from the CUG816 codon within the 5’UTR region of PTEN mRNA. PTENε mainly localizes in the cell membrane, and physically associates with and dephosphorylates VASP and ACTR2, which govern filopodia formation and cell motility. We found that endogenous depletion of PTENε promotes filopodia formation and enhances the metastasis capacity of tumor cells. Overall, we identify a new isoform of PTEN with distinct localization and function compared to the known members of the PTEN family. These findings enrich the PTEN family constitution and advance our current understanding of the importance and diversity of PTEN functions.
HostingRepository	PRIDE
AnnounceDate	2021-01-11
AnnouncementXML	Submission_2021-01-10_23:58:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	张 巧玲
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-29 04:12:27	ID requested
⏵ 1	2021-01-10 23:58:22	announced

Dataset with its publication pending

ProteomeXchange project tag: Cancer (B/D-HPP), Human Proteome Project

submitter keyword: Alternative initiation/Filopodia formation/PTENε/PTEN isoform/Metastasis.

Qiaoling Zhang
contact affiliation	Institute of Systems Biomedicine, Department of Pathology, School of Basic Medical Sciences, Beijing Key Laboratory of Tumor Systems Biology, Peking-Tsinghua Center of Life Sciences, Peking University Health Science Center, Beijing 100191, China.
contact email	zql1210305164@bjmu.edu.cn
lab head
张 巧玲
contact affiliation	peking university
contact email	zql1210305164@bjmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022245

PRIDE project URI

• PRIDE
  1. PXD022245
     1. Label: PRIDE project
     2. Name: PTENε, a new isoform of PTEN, suppresses tumor metastasis through filopodia formation regulation.