PXD022189

PXD022189 is an original dataset announced via ProteomeXchange.

Title	Label-Free LC-MS/MS Proteomic Analysis of Urinary Identification in Diabetic Vascular Dementia
Description	Objective: The aim of this study was to identify novel diagnostic biomarkers of diabetic vascular dementia (DVD) and unraveling the underlying mechanisms using mass spectrometry (MS). Methods: Label-Free LC-MS/MS proteomics analysis was applied to urine samples from four groups including 14 vascular dementia CC(VD), 22 type 2 diabetes mellitus (T2DM), 12 DVD, 21 Normal Control (NC). Searching the MS data by Proteome Discoverer software, protein abundances were analyzed qualitatively and quantitatively and compared between these groups. Combining bioinformatics analysis using GO, pathway crosstalk analysis using KEGG, PPI network analysis using STRING and literature searching, the differentially expressed proteins (DEPs) of DVD can be comprehensively judged and were further quantified by receiver operating characteristic (ROC) curve methods. Results: The proteomic findings showed quantitative changes in DVD was compared to NC, T2DM, and VD groups; among 7,527 identified urine proteins, 1222, 1152, and 1180 of the proteins displayed quantitative changes unique to DVD vs NC, T2DM, and VD, respectively, including 481 overlapped common DEPs. Then 9 unique proteins and 2 composite markers (CM) were confirmed by a ROC curve method. Conclusion: This study provided a novel insight into the potential pathogenesis of DVD and elucidated a method for the earlier detection.
HostingRepository	PRIDE
AnnounceDate	2021-01-14
AnnouncementXML	Submission_2021-01-13_23:58:31.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi Zeng
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-26 07:53:46	ID requested
⏵ 1	2021-01-13 23:58:31	announced

Dataset with its publication pending

submitter keyword: diabetes, vascular dementia, biomarkers, protein, urine, proteomics

Yi Zeng
contact affiliation	second xiangya hospital central south university
contact email	zengyi_xyneuro@csu.edu.cn
lab head
Yi Zeng
contact affiliation	Second Xiangya Hospital, Central South University
contact email	zengyi_xyneuro@csu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022189

PRIDE project URI

• PRIDE
  1. PXD022189
     1. Label: PRIDE project
     2. Name: Label-Free LC-MS/MS Proteomic Analysis of Urinary Identification in Diabetic Vascular Dementia