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PXD022165

PXD022165 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic insights into senescence of testicular peritubular cells from a nonhuman primate model
DescriptionAge-related changes of the human testis may include morphological alterations, disturbed steroidogenesis and impaired spermatogenesis. However, the specific impact of cell age remains poorly understood and difficult to assess. Testicular peritubular cells fulfill essential functions, including sperm transport, contributions to the spermatogonial stem cell niche and paracrine interactions within the testis. To study their role in age-associated decline of testicular functions, we performed comprehensive proteome and secretome analyses of repeatedly passaged peritubular cells from Callithrix jacchus. This nonhuman primate model better reflects the human testicular biology than rodents and further gives access to young donors unavailable from humans. Among 5095 identified proteins, 583 were differentially abundant between samples with low and high passage numbers. The alterations indicate a reduced ability of senescent peritubular cells to contract and secrete proteins, as well as disturbances in NF-κB signaling and a reduced capacity to handle reactive oxygen species. Since this in vitro model may not exactly mirror all molecular aspects of in vivo aging, we investigated the proteomes and secretomes of testicular peritubular cells from young and old donors. Even though the age-related alterations at the protein level were less pronounced, we found evidence for impaired protein secretion, altered NF-κB signaling and reduced contractility of these in vivo aged peritubular cells.
HostingRepositoryPRIDE
AnnounceDate2020-11-18
AnnouncementXMLSubmission_2020-11-18_02:58:37.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJan Bernd Stöckl
SpeciesList scientific name: Callithrix jacchus; NCBI TaxID: 9483;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-26 01:10:46ID requested
12020-11-18 02:58:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Ageing, testis, cellular model, proteome, marmoset monkey, non-human primate, senescence, secretome
Contact List
Thomas Fröhlich
contact affiliationLaboratory for Functional Genome Analysis LAFUGA, Gene Center, LMU München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
contact emailfrohlich@genzentrum.lmu.de
lab head
Jan Bernd Stöckl
contact affiliationGenzentrum der LMU München
contact emailstoeckl@genzentrum.lmu.de
dataset submitter
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Dataset FTP location
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