PXD022135

PXD022135 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	USP13 interacts with cohesin and is required for its ubiquitination in human cells
Description	Gene editing was used to modify an endogenous allele of USP13 in human HCT116 cells with the addition of a 1X FLAG/Streptavidin Binding Peptide dual epitope tag. Nuclear lysates were prepared and dual affinity purification was performed. Then, GelC/MS/MS was performed to identify proteins that were present after dual affinity purification from dual epitope-tagged cells but not from dual affinity purification from untagged parental cells.
HostingRepository	MassIVE
AnnounceDate	2020-10-22
AnnouncementXML	Submission_2020-10-22_10:47:51.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bogdan Budnik
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; Deamidated
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-22 09:39:39	ID requested
⏵ 1	2020-10-22 10:47:51	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: deubiquitination, chromatin, cell cycle, cohesin, USP13

submitter keyword: deubiquitination, chromatin, cell cycle, cohesin, USP13

Contact List

Todd Waldman
contact affiliation	Georgetown University School of Medicine
contact email	waldmant@georgetown.edu
lab head
Bogdan Budnik
contact affiliation	Harvard University
contact email	bbudnik@mcb.harvard.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086356/