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PXD022067

PXD022067 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes
DescriptionPost-translational methylation plays a crucial role in regulating and optimizing protein function. Protein histidine methylation, occurring as the two isomers 1- and 3- methylhistidine (1MH and 3MH), was first reported five decades ago, but remains largely unexplored. Here we report that METTL9 is a broad-specificity methyltransferase (MTase) that mediates the formation of the majority of 1MH present in mouse and human proteomes. The minimal requirement for METTL9-catalyzed methylation is a His-x-His (HxH) motif, where "x" is preferably a small amino acid, allowing METTL9 to methylate a number of HxH-containing proteins. Here, we show that the immunomodulatory protein S100A9 and the NDUFB3 subunit of mitochondrial respiratory Complex I are methylated at HxH motif using MALDI-TOF MS.
HostingRepositoryPRIDE
AnnounceDate2020-11-23
AnnouncementXMLSubmission_2020-11-23_07:28:14.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD022067
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTakehiro Suzuki
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidoethyl-L-cysteine; monohydroxylated residue; 1'-methyl-L-histidine
InstrumentBruker Daltonics flex series
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-19 03:54:12ID requested
12020-11-23 07:28:14announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Metylation Histidine METTL9
Contact List
Naoshi Dohmae
contact affiliationBiomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact emaildohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliationRIKEN Center for Sustainable Resource Science
contact emailtakehirosuzuki@riken.jp
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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