PXD022019

PXD022019 is an original dataset announced via ProteomeXchange.

Title	The influence of delay in mononuclear cell isolation on acute myeloid leukemia phosphorylation profiles
Description	Phosphorylation is a dynamic, highly regulated post-translational modification that may be affected by variation in pre-analytical sample handling, hampering the translational value of phosphoproteomic-based data. Here, we investigate the temporal effect of delay in mononuclear cell isolation on acute myeloid leukemia (AML) phosphorylation profiles. We performed MS on immuno-precipitated phosphotyrosine (pY)-containing peptides isolated from AML samples after seven pre-defined delays before sample processing (direct processing, thirty minutes, one hour, two hours, three hours, four hours and 24 hours delay). Up to four hours, phosphorylation profiles showed limited variation. However, in samples processed with a delay of 24 hours, we observed significant change in phosphorylation profiles, with differential phosphorylation of 22 phosphopeptides (p < 0.01). This included increased phosphorylation of phosphopeptides of JNK and p38 kinases indicative of stress response activation. Based on these results, we conclude that processing of AML samples should be standardized at all times and should occur within four hours after sample collection.
HostingRepository	PRIDE
AnnounceDate	2021-02-22
AnnouncementXML	Submission_2021-02-22_05:57:39.679.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sander Piersma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-16 01:25:24	ID requested
⏵ 1	2021-02-22 05:57:40	announced

Dataset with its publication pending

submitter keyword: Acute myeloid leukemia, AML, phosphoproteomics, phosphotyrosine, ischemia, oxidative stress, pre-analytical variables, tyrosine kinase

Connie Ramona Jimenez
contact affiliation	OncoProteomics Laboratory, Dept of Medical Oncology, VU University Medical Center, Amsterdam, The Netherlands
contact email	c.jimenez@vumc.nl
lab head
Sander Piersma
contact affiliation	Amsterdam UMC
contact email	s.piersma@amsterdamumc.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD022019

PRIDE project URI

• PRIDE
  1. PXD022019
     1. Label: PRIDE project
     2. Name: The influence of delay in mononuclear cell isolation on acute myeloid leukemia phosphorylation profiles