PXD021826

PXD021826 is an original dataset announced via ProteomeXchange.

Title	Mitochondria-rough-ER Contacts in The Liver Regulate Systemic Lipid Homeostasis
Description	Contacts between organelles create microdomains that play major roles in regulating key intracellular activities and signaling pathways, but whether they also directly regulate systemic cellular activities, remains unknown. Here, we report the ultrastructural organization and dynamics of a new type of inter-organelle contact which is formed by rough-Endoplasmic Reticulum that is closely wrapped around the mitochondrion (wrappER). To elucidate the in vivo function of this inter-organelle association, mouse liver fractions enriched in wrappER-associated-mitochondria were analyzed in parallel by transcriptomics, proteomics, and lipidomics. The biochemical signature of the wrappER points to an unexpected role in the biogenesis of very-low-density lipoproteins (VLDLs). Altering wrappER-mitochondria contacts curtails liver VLDL secretion and increases hepatic fatty acids, lipid droplets and neutral lipid content. Conversely, acute liver-specific ablation of Mttp, the most upstream regulator of VLDL biogenesis, mirrors this hepatic dyslipidemia phenotype and promotes remodelling of the wrappER-mitochondria contact. The participation of liver wrappER-mitochondria contacts in VLDL biology reveals a role of inter-organelle contacts in systemic lipid homeostasis.
HostingRepository	PRIDE
AnnounceDate	2021-03-15
AnnouncementXML	Submission_2021-03-15_08:10:30.785.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD021826
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Luca Pellegrini
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	deaminated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	SCIEX instrument model

Revision	Datetime	Status	ChangeLog Entry
0	2020-10-05 03:51:20	ID requested
⏵ 1	2021-03-15 08:10:31	announced

Dataset with its publication pending

submitter keyword: Mouse, Liver, wrappER, WAM, Lipid Homeostasis, VLDL

Luca Pellegrini
contact affiliation	Department of Molecular biology, medical biochemistry and pathology, Faculty of Medicine, Laval University, Quebec, QC, Canada
contact email	luca.pellegrini@fmed.ulaval.ca
lab head
Luca Pellegrini
contact affiliation	Université Laval
contact email	luca.pellegrini@fmed.ulaval.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021826
     1. Label: PRIDE project
     2. Name: Mitochondria-rough-ER Contacts in The Liver Regulate Systemic Lipid Homeostasis