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PXD021802

PXD021802 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title Characterization and Complete Genome Sequence of Privateer, a Highly Prolate Proteus mirabilis Podophage
DescriptionThe Gram-negative bacterium Proteus mirabilis causes a large proportion of catheter-associated urinary tract infections (CAUTIs), which are among the world's most common nosocomial infections. Here, we characterize P. mirabilis bacteriophage Privateer, a prolate podophage of the C3 morphotype isolated from Texas wastewater treatment plant activated sludge. Basic characterization assays demonstrated Privateer has a latent period of ~40 minutes and average burst size around 100. In the 90.7 kb Privateer genome, 43 functions were assigned for the 144 predicted protein-coding genes. Genes encoding DNA replication proteins, DNA modification proteins, four tRNAs, lysis proteins, and structural proteins were identified. Cesium-gradient purified Privateer particles analyzed via LC-MS/MS verified the presence of several predicted structural proteins, including the major capsid protein and a longer, minor capsid protein apparently produced by translational frameshift. Comparative analysis demonstrated Privateer shares 83% nucleotide similarity with Cronobacter phage vB_CsaP_009, but low nucleotide similarity with other known phages. Predicted structural proteins in Privateer appear to have evolutionary relationships with other prolate podophages, in particular the Kuraviruses.
HostingRepositoryMassIVE
AnnounceDate2020-11-29
AnnouncementXMLSubmission_2020-11-29_15:45:11.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSusan T. Weintraub
SpeciesList scientific name: Proteus phage Privateer;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-10-02 13:21:36ID requested
12020-11-29 15:45:12announced
Publication List
no publication
Keyword List
submitter keyword: bacteriophage, genomics, proteus
Contact List
Ryland Young, Ph.D.
contact affiliationTexas A&M University
contact emailryland@tamu.edu
lab head
Susan T. Weintraub
contact affiliationUT Health Science Center San Antonio
contact emailweintraub@uthscsa.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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