PXD021728

PXD021728 is an original dataset announced via ProteomeXchange.

Title	Caspase-3 cleaves extracellular vesicle proteins during auditory brainstem development
Description	Sound localization requires extremely precise development of auditory brainstem circuits, the molecular mechanisms of which are largely unknown. We previously demonstrated a novel requirement for non-apoptotic activity of the protease caspase-3 in chick auditory brainstem development. Here, we used mass spectrometry to identify proteolytic substrates of caspase-3 during chick auditory brainstem development. Functional annotation analysis revealed that our caspase-3 substrates were enriched more than two-fold for proteins associated with extracellular vesicles (EVs), membrane-bound nanoparticles that function in intercellular communication. The proteome of EVs isolated from the auditory brainstem contained caspase-3 and was highly enriched for the caspase-3 substrates identified here. Additionally, we identified two caspase-3 substrates with known functions in axon guidance, namely Neural Cell Adhesion Molecule (NCAM) and Neuronal-glial Cell Adhesion Molecule (Ng-CAM), that were found in auditory brainstem EVs and expressed in the auditory pathway alongside cleaved caspase-3. Taken together, these data suggest a novel developmental mechanism whereby caspase-3 influences auditory brainstem circuit formation through the proteolytic cleavage of EV proteins.
HostingRepository	PRIDE
AnnounceDate	2020-10-16
AnnouncementXML	Submission_2020-10-16_00:32:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Forrest Weghorst
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-28 23:30:18	ID requested
⏵ 1	2020-10-16 00:32:55	announced

Dataset with its publication pending

submitter keyword: Caspase-3, chicken, neurodevelopment, brainstem

Karina Cramer
contact affiliation	Department of Neurobiology and Behavior, University of California, Irvine
contact email	cramerk@uci.edu
lab head
Forrest Weghorst
contact affiliation	Department of Neurobiology and Behavior, University of California, Irvine
contact email	fweghors@uci.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD021728

PRIDE project URI

• PRIDE
  1. PXD021728
     1. Label: PRIDE project
     2. Name: Caspase-3 cleaves extracellular vesicle proteins during auditory brainstem development