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PXD021673

PXD021673 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS/MS analysis of lesional and normally looking psoriatic skin
DescriptionBackground: Plaque psoriasis is a chronic autoimmune disorder characterized by the development of red scaly plaques. To date psoriasis lesional skin transcriptome has been extensively studied, whereas only few proteomic studies of psoriatic skin are available. Aim: The aim of this study was to compare protein expression patterns of lesional and normally looking skin of psoriasis patients with skin of the healthy volunteers, reveal differentially expressed proteins and identify changes in cell metabolism caused by the disease. Methods: Skin samples of normally looking and lesional skin donated by psoriasis patients (n = 5) and samples of healthy skin donated by volunteers (n = 5) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). After protein identification and data processing, the set of differentially expressed proteins was subjected to protein ontology analysis to characterize changes in biological processes, cell components and molecular functions in the patients' skin compared to skin of the healthy volunteers. Results: The performed analysis identified 405 and 59 differentially expressed proteins in lesional and normally looking psoriatic skin compared to healthy control. We discovered decreased expression of KNG1, APOE, HRG, THBS1 and PLG in normally looking skin of the patients. Presumably, these changes were needed to protect the epidermis from spontaneous activation of kallikrein-kinin system and delay the following development of inflammatory response. In lesional skin, we identified several large groups of proteins with coordinated expression. Mainly, these proteins were involved in different aspects of protein and RNA metabolism, namely ATP synthesis and consumption; intracellular trafficking of membrane-bound vesicles, pre-RNA processing, translation, chaperoning and degradation in proteasomes/immunoproteasomes. Conclusion: Our findings explain the molecular basis of metabolic changes caused by disease in skin lesions, such as faster cell turnover and higher metabolic rate. They also indicate on downregulation of kallikrein-kinin system in normally looking skin of the patients that would be needed to delay exacerbation of the disease.
HostingRepositoryPRIDE
AnnounceDate2021-05-06
AnnouncementXMLSubmission_2021-05-05_21:53:53.888.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRustam Ziganshin
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-24 07:26:10ID requested
12021-05-05 21:53:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LC-MS/MS, proteome, plaque psoriasis
Contact List
Oxana A. Svitich
contact affiliationLaboratory of Molecular Immunology, I. Mechnikov Research Institute for Vaccines and Sera Director of the I. Mechnikov Research Institute for Vaccines and Sera
contact emailsvitichoa@yandex.ru
lab head
Rustam Ziganshin
contact affiliationShemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences
contact emailrustam.ziganshin@gmail.com
dataset submitter
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